Comparison of monoclonal antibody and calcofluor white stains for the detection of Pneumocystis carinii from respiratory specimens
نویسندگان
چکیده
منابع مشابه
Comparison of monoclonal antibody and calcofluor white stains for the detection of Pneumocystis carinii from respiratory specimens.
Three monoclonal antibody staining kits, from Genetic Systems, Disease Detection International, and Meridian Diagnostics, were compared with calcofluor white for the direct detection of Pneumocystis carinii in respiratory specimens. Of the 150 specimens tested, 23 were found positive for P. carinii by any of the four stains; 13 were bronchoalveolar lavage, 7 were induced sputum and 3 were expec...
متن کاملComparison of four methods for rapid detection of Pneumocystis carinii in respiratory specimens.
Four stains for the detection of Pneumocystis carinii in respiratory specimens were compared for sensitivity, specificity, preparation time, and ease of interpretation. One hundred specimens were collected. Of these, 50 were induced sputum specimens and 50 were bronchoalveolar lavage fluid. All specimens were stained with Diff-Quik (DQ) (a modified Giemsa stain), a quick silver stain, and direc...
متن کاملPerformance of a PCR assay for detection of Pneumocystis carinii from respiratory specimens.
This study evaluates the performance of a PCR assay for the detection of Pneumocystis carinii from respiratory specimens that has been designed for use in the clinical microbiology laboratory. The test includes a simple method for nucleic acid extraction and amplification, a colorimetric probe hybridization technique for detection of amplicons, and an internal control to evaluate for the presen...
متن کاملMouse Anti-Pneumocystis carinii Monoclonal Antibody
carinii. Specificity: QSP228 antibody reacts specifically with P. carinii pneumonia, staining especially cysts present in biopsy material, bronchial lavage and induced sputum samples. For bronchial lavage and induced sputum samples pretreatment of samples with 0.3% dithiothreitol or sputolysin is recommended. In Western blotting analysis the antibody identifies an antigen of 93 kD of P. carinii...
متن کامل"rapid detection of pneumocystis carini in spiratory specimens of rats by calcofluor white staining"
the present study was carried out for evaluation of calcoflour white staining (cws) as a rapid method for detection of pneumocystis carinii in respiratory specimens of rats as an animal model for human infection. a total of 35 spraque – dawley rats were divided into two groups. group1 (20 rats) received increasing doses of dexamethasone subcutaneously, and group 2(15 rats)as control group that ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1991
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.29.3.645-647.1991